Mgr. Lukáš Pečinka, Ph.D.
Junior Researcher
Cell and Tissue Regeneration
Our team investigates fundamental mechanisms and translates findings from bench to bedside, combining preclinical models with clinical insight.
Researcher at ICRC with focus on translational medicine. Combines preclinical insights with clinical practice across cardiology, neurology, and oncology programs.
Currently leads / contributes to interdisciplinary projects bridging molecular biology, imaging, and patient care at St. Anne's University Hospital Brno.
Research Group
- Team size
- Team size: 12 Researchers
- Place
- Place: Building D3, Ground Floor
- Collaborates with
- Collaborates with Mayo Clinic, USA
Head of Research Group
- prof. MVDr. Aleš Hampl, CSc.
Members
MUDr. Klára Koudelková
Research Assistant
Andrea Koláčková
Research Assistant
Mgr. Dáša Bohačiaková, Ph.D.
Researcher
Mgr. Petr Fojtík, Ph.D.
Researcher
Mgr. Jan Raška, Ph.D.
Researcher
Mgr. Jana Slováčková, Ph.D.
Researcher
MVDr. Anna Mac Gillavry Danylevska, Ph.D.
Research Technician, Postdoctoral Researcher
Mgr. Hana Kotasová, Ph.D.
Senior Postdoctoral Researcher
doc. RNDr. Petr Vaňhara, Ph.D.
Senior Postdoctoral Researcher
prof. MVDr. Aleš Hampl, CSc.
Senior Research Team Leader
Team Assistant
Bc. Ivana Hanáková
Administrative Support
Main Objectives
- Establishment of clinical grade (GMP quality) lines of human embryonic stem cells and their functional evaluation.
- Understanding and manipulating biological properties of different types of human pluripotent stem cells (tissue, embryonic, induced) that are relevant to their safe application in clinics.
Research focus
We investigate the properties of different types of human stem cells that are relevant to their utility in biomedicine
Particular focus is on pluripotent stem cells, both embryonic and induced, but adult stem cell types are also studied. The biological phenomena that are being studied mainly include:
genetic and genomic stability of stem cells because of its importance to safety in potential clinical applications
role of cell cycle regulators and non-coding RNAs in establishing and maintenance of differentiated phenotypes and also in pluripotency
effects of extracellular biodomains on behavior of stem cells, with an emphasis on their contribution to establishment of niche environment.
Great effort is also dedicated to:
development of methodologies for supporting growth of cells in engineered biocompatible scaffolds
differentiation of stem cells towards defined somatic cell lineages (e.g. airway epithelia, neural crest derivatives).
development of technologies important for production of cell products under cGMP standards
Lastly, the group is involved in setting standards and rules related to ethical and safe use of stem cells in clinics, via its participation in International Stem Cell Forum and International Stem Cell Banking Initiative, Karolinska Institutet, Stockholm, Sweden, University of Newcastle, Newcastle, UK and Stem Cell Institute, MRC, Cambridge, UK
Technological equipment
- BioScaffolder 3.1 (GESIM, Germany) – printing of wide variety of 3D structures in micrometer dimensions, also with live cells.
- VEVO 2100 – high-frequency, high-resolution digital imaging platform with linear array technology and Color Doppler Mode for small animals.
- ImageXpress Micro XL (Molecular Devices, USA) – epifluorescence live cell imaging microscopy with high content screening capability.
Selected Results
- Kotasova, H., M. Capandova, V. Pelkova, J. Dumkova, Z. Koledova, J. Remsik, K. Soucek, Z. Garlikova, V. Sedlakova, A. Rabata, P. Vanhara, L. Moran, L. Pecinka, V. Porokh, M. Kucirek, L. Streit, J. Havel and A. Hampl (2022). “Expandable Lung Epithelium Differentiated from Human Embryonic Stem Cells.” Tissue Eng Regen Med.
- Lenart, S., P. Lenart, L. Knopfova, H. Kotasova, V. Pelkova, V. Sedlakova, O. Vacek, J. Pokludova, V. Can, J. Smarda, K. Soucek, A. Hampl and P. Benes (2022). “TACSTD2 upregulation is an early reaction to lung infection.” Sci Rep 12(1): 9583.
- Vazquez-Gomez, G., M. Karasova, Z. Tylichova, M. Kabatkova, A. Hampl, J. Matthews, J. Neca, M. Ciganek, M. Machala and J. Vondracek (2022). “Aryl Hydrocarbon Receptor (AhR) Limits the Inflammatory Responses in Human Lung Adenocarcinoma A549 Cells via Interference with NF-kappaB Signaling.” Cells 11(4).
- Weissova, K., B. Fafilek, T. Radaszkiewicz, C. Celiker, P. Machackova, T. Cechova, J. Sebestikova, A. Hampl, V. Bryja, P. Krejci and T. Barta (2022). “LuminoCell: a versatile and affordable platform for real-time monitoring of luciferase-based reporters.” Life Sci Alliance 5(8).
- Golunova, A., N. Velychkivska, Z. Miksovska, V. Chochola, J. Jaros, A. Hampl, O. Pop-Georgievski and V. Proks (2021). “Direct and Indirect Biomimetic Peptide Modification of Alginate: Efficiency, Side Reactions, and Cell Response.” Int J Mol Sci 22(11).
- Kolarova, H., J. Vitecek, A. Cerna, M. Cernik, J. Pribyl, P. Skladal, D. Potesil, I. Ihnatova, Z. Zdrahal, A. Hampl, A. Klinke and L. Kubala (2021). “Myeloperoxidase mediated alteration of endothelial function is dependent on its cationic charge.” Free Radic Biol Med 162: 14-26.
- Souralova, T., Z. Holubcova, D. Kyjovska, A. Hampl and I. Koutna (2021). “Xeno- and feeder-free derivation of two sex-discordant sibling lines of human embryonic stem cells.” Stem Cell Res 57: 102574.
- Trebichalska, Z., J. Javurek, M. Tatickova, D. Kyjovska, S. Kloudova, P. Otevrel, A. Hampl and Z. Holubcova (2021). “High-Resolution 3D Reconstruction of Human Oocytes Using Focused Ion Beam Scanning Electron Microscopy.” Front Cell Dev Biol 9: 755740.
- Trebichalska, Z., D. Kyjovska, S. Kloudova, P. Otevrel, A. Hampl and Z. Holubcova (2021). “Cytoplasmic maturation in human oocytes: an ultrastructural study dagger.” Biol Reprod 104(1): 106-116.
- Ventruba, P., J. Zakova, M. Jeseta, E. Lousova, A. Hampl, T. Souralova and I. Koutna (2021). “Aspects of embryo selection and their preparation for the formation of human embryonic stem cells intended for human therapy.” Ceska Gynekol 86(1): 5-10.
